Journal: Precision Clinical Medicine

Article Title: Agrimol B inhibits pancreatic ductal adenocarcinoma by induction of lethal mitophagy through decreasing mitochondrial transcription termination factor 3

doi: 10.1093/pcmedi/pbag009

Figure Lengend Snippet: Agrimol B sensitizes PDAC cells to first-line chemotherapy drugs. (A) Schematic overview of PDAC PDO establishment and drug assessment. (B, C) Brightfield images of organoids treated with the indicated concentrations of Agrimol B. Scale bars, 10 μm. (D) Chemical structures of nab-paclitaxel (Paclitaxel), irinotecan, 5-fluorouracil, oxaliplatin, and gemcitabine. (E–H) Brightfield images of organoids treated with or without Agrimol B in the presence or absence of nab-paclitaxel, irinotecan, 5-fluorouracil, oxaliplatin, or gemcitabine. Scale bars, 10 μm.

Article Snippet: Hydroxychloroquine (HCQ) (HY-W031727), wortmannin (WORT) (HY-10197), Mdivi-1 (HY-15886), N-acetylcysteine (HY-B0215), Nab-Paclitaxel (HY-P99974), irinotecan (HY-16562), 5-fluorouracil (5-FU) (HY-107856), and oxaliplatin (HY-17371) were purchased from MedChem Express.

Techniques:

Journal: RSC Advances

Article Title: pH-responsive sodium alginate/CMCS/CMCNa composite hydrogel beads for sustained delivery of 5-fluorouracil-β-cyclodextrin inclusion complexes

doi: 10.1039/d6ra00194g

Figure Lengend Snippet: (a) Synthesis of β-CD@5-Fu; (b) preparation process of β-CD@5-Fu/CMCS/CMCNa/SA hydrogel beads; (c) in vitro release mechanism of β-CD@5-Fu/CMCS/CMCNa/SA hydrogel beads.

Article Snippet: β-Cyclodextrin (β-CD) and sodium alginate (SA) were purchased from Shanghai Aladdin Biochemical Technology, Carboxymethyl chitosan (CMCS), carboxymethyl cellulose sodium (CMCNa), calcium chloride (CaCl 2 ), and sodium chloride (NaCl) were obtained from Sinopharm Chemical Reagent, 5-fluorouracil (5-Fu) was acquired from Shanghai Macklin Biochemical Technology.

Techniques: In Vitro

Journal: RSC Advances

Article Title: pH-responsive sodium alginate/CMCS/CMCNa composite hydrogel beads for sustained delivery of 5-fluorouracil-β-cyclodextrin inclusion complexes

doi: 10.1039/d6ra00194g

Figure Lengend Snippet: Characterization of β-CD@5-Fu inclusion complex and drug-loaded hydrogels: (a) UV-Vis spectra of 5-Fu, β-CD, and β-CD@5-Fu; (b) FTIR spectra of 5-Fu, β-CD, and β-CD@5-Fu; (c) XRD patterns of 5-Fu, β-CD, and β-CD@5-Fu; (d) UV-Vis calibration curves of 5-Fu at different concentrations; (e) linear regression of 5-Fu concentration versus absorbance; (f) encapsulation efficiency (EE) and drug loading (DL) capacity of β-CD@5-Fu; (g) absorbance comparison of β-CD@5-Fu in water versus PBS; (h and i) EE and DL of hydrogel beads with different drug-carrier ratios (w/w).

Article Snippet: β-Cyclodextrin (β-CD) and sodium alginate (SA) were purchased from Shanghai Aladdin Biochemical Technology, Carboxymethyl chitosan (CMCS), carboxymethyl cellulose sodium (CMCNa), calcium chloride (CaCl 2 ), and sodium chloride (NaCl) were obtained from Sinopharm Chemical Reagent, 5-fluorouracil (5-Fu) was acquired from Shanghai Macklin Biochemical Technology.

Techniques: Concentration Assay, Encapsulation, Comparison

Journal: RSC Advances

Article Title: pH-responsive sodium alginate/CMCS/CMCNa composite hydrogel beads for sustained delivery of 5-fluorouracil-β-cyclodextrin inclusion complexes

doi: 10.1039/d6ra00194g

Figure Lengend Snippet: Simultaneous thermogravimetry-differential scanning calorimetry (TG-DSC) curves of 5-Fu (a), β-CD (b), physical mixture of 5-Fu and β-CD (c), β-CD@5-Fu inclusion complex (d), CMCS/CMCNa/SA hydrogel beads (e), and β-CD@5-Fu/CMCS/CMCNa/SA (f).

Article Snippet: β-Cyclodextrin (β-CD) and sodium alginate (SA) were purchased from Shanghai Aladdin Biochemical Technology, Carboxymethyl chitosan (CMCS), carboxymethyl cellulose sodium (CMCNa), calcium chloride (CaCl 2 ), and sodium chloride (NaCl) were obtained from Sinopharm Chemical Reagent, 5-fluorouracil (5-Fu) was acquired from Shanghai Macklin Biochemical Technology.

Techniques: Differential Scanning Calorimetry

Journal: RSC Advances

Article Title: pH-responsive sodium alginate/CMCS/CMCNa composite hydrogel beads for sustained delivery of 5-fluorouracil-β-cyclodextrin inclusion complexes

doi: 10.1039/d6ra00194g

Figure Lengend Snippet: (a) FTIR spectra of raw materials; (b) FTIR spectra of six hydrogel bead formulations; swelling ratio of β-CD@5-Fu/CMCS/CMCNa/SA hydrogel dried beads (c) and swollen beads (d) in purified water and under different pH conditions (pH 1.2; pH 6.8 with and without phosphate; pH 7.4 with and without phosphate) at 37 °C for 24 h.

Article Snippet: β-Cyclodextrin (β-CD) and sodium alginate (SA) were purchased from Shanghai Aladdin Biochemical Technology, Carboxymethyl chitosan (CMCS), carboxymethyl cellulose sodium (CMCNa), calcium chloride (CaCl 2 ), and sodium chloride (NaCl) were obtained from Sinopharm Chemical Reagent, 5-fluorouracil (5-Fu) was acquired from Shanghai Macklin Biochemical Technology.

Techniques: Purification

Journal: RSC Advances

Article Title: pH-responsive sodium alginate/CMCS/CMCNa composite hydrogel beads for sustained delivery of 5-fluorouracil-β-cyclodextrin inclusion complexes

doi: 10.1039/d6ra00194g

Figure Lengend Snippet: Scanning electron microscopy (SEM) images of hydrogel beads with different formulations: (a–f) β-CD@5-Fu-loaded hydrogel beads with varying mass ratios of β-CD@5-Fu to CCS: (a and b) 20 : 30, (c and d) 15 : 35, and (e and f) 10 : 40. (g–l) 5-Fu-incorporated hydrogel beads (without β-CD) at corresponding ratios: (g and h) 20 : 30, (i and j) 15 : 35, and (k and l) 10 : 40. (m–p) Control groups: (m and n) pure SA hydrogel beads and (o and p) CMCS/CMC-Na/SA ternary hydrogel beads (without drugs).

Article Snippet: β-Cyclodextrin (β-CD) and sodium alginate (SA) were purchased from Shanghai Aladdin Biochemical Technology, Carboxymethyl chitosan (CMCS), carboxymethyl cellulose sodium (CMCNa), calcium chloride (CaCl 2 ), and sodium chloride (NaCl) were obtained from Sinopharm Chemical Reagent, 5-fluorouracil (5-Fu) was acquired from Shanghai Macklin Biochemical Technology.

Techniques: Electron Microscopy, Control

Journal: RSC Advances

Article Title: pH-responsive sodium alginate/CMCS/CMCNa composite hydrogel beads for sustained delivery of 5-fluorouracil-β-cyclodextrin inclusion complexes

doi: 10.1039/d6ra00194g

Figure Lengend Snippet: Drug release performance: (a) release kinetics; (b) shows the comparison results of the cumulative release rate (CFR) of six different hydrogel beads; (c and d) in vitro release of β-CD@5-Fu and 5-Fu loaded hydrogels at different ratios (the error bars represent the standard deviation of three replicate experiments); (e) structural stability in SGF (0–2 h) and SIF (0–12 h); (f) schematic diagram of the drug release mechanism from hydrogel beads.

Article Snippet: β-Cyclodextrin (β-CD) and sodium alginate (SA) were purchased from Shanghai Aladdin Biochemical Technology, Carboxymethyl chitosan (CMCS), carboxymethyl cellulose sodium (CMCNa), calcium chloride (CaCl 2 ), and sodium chloride (NaCl) were obtained from Sinopharm Chemical Reagent, 5-fluorouracil (5-Fu) was acquired from Shanghai Macklin Biochemical Technology.

Techniques: Comparison, In Vitro, Standard Deviation